Detection of Verotoxigenic Escherichia coli by Magnetic Capture-Hybridization PCR
نویسندگان
چکیده
منابع مشابه
rapid detection of escherichia coli o157: h7 by fluorescent amplification–based specific hybridization (flash) pcr
background escherichia coli o157:h7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. methods we developed fluorescent amplification-based specific hybridization (flash–pcr) assay to detect the stx-encoding gene stx-1 of e. coli o157:h7. result pcr product of 336 bp was suc...
متن کاملRapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
BACKGROUND Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS We developed fluorescent amplification-based specific hybridization (FLASH-PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7. RESULT PCR product of 336 bp was...
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, although no specific lesions compatible with this infectious agent were observed. A pool containing all morbillivirus-positive PCR amplicons for animals 1 and 2 (GenBank accession nos. KT006289 and KT006290), a PCR amplicon for the brain sample from animal 2 (GenBank accession no. KT006291), and a PCR amplicon for the larynx from animal 3 were se-quenced. A BLAST search (http://www.ncbi.nlm.n...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1998
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.64.1.147-152.1998